Module Aims
(minimum 210 characters) |
The course will introduce the students to selected up-to-date diagnostic and therapeutic techniques and methods in molecular medicine. Major topics include molecular genetic and cell biology methods in the clinical practice, genomic and proteomic techniques, cell and tissue culture methods. Diagnostic methods based on immunologic and nucleic acid hybridization will be discussed. A series of lectures will deal with cell signalization processes and the regulation of gene expression. Gene expresion profiling and its diagnostic value will be discussed. Embryonal and adult stem cells and their possible therapeutic use, as well as telomerase-directed molecular therapy, immunotherapy and antitumour immune responses will be discussed in detail. |
Module Subject
(minimum 350 characters) |
1) Molecular genetic and cell biology methods in the clinical practice. Diagnostic methods based on immunologic techniques (RIA, ELISA, Western blot analysis, immunocytology, etc.).
2) Diagnostic methods based nucleic acid hybridization (Northern blot analysis, in situ hybridization, DNA chip technology, etc.). Gene sequencing and analysis, genomic and proteomic techniques, cell and tissue culture methods.
3) Molecular markers in human disorders. Diagnostic methods based on specific endonuclease activity (fragment length polymorphism, pedigree analysis, etc.). Molecular biological methods in clinical practice.
4) Intracellular signalization in pathophysiological processes.
5) Regulation of gene expression.
6) Regulation of neuronal gene expression.
7) Molecular biological aspects of neurologic and psychiatric disorders.
8) Gene therapy, viral vectors, DNA-liposome complexes.
9) Antisense pharmacology. Small interfering RNA. Molecular chaperons.
10) Embryonal and adult stem cells.
11) Neuronal stem cells. The feasibility of stem cell therapies.
12) In vitro differentiation of stem cells to the desired phenotype. Transfection of stem cells.
13) Regulation of cell cycle and cell differentiation, translation control of gene expression.
14) Telomerase-directed molecular therapy. Immunotherapy. Antitumour immune responses.
15) Bioinformatic and computer-assisted methods in diagnosis and therapy: functional genomics and proteomics.
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